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Below are our current protease-related job listings:

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Xilio Therapeutics is a development-stage biotechnology company advancing next-generation cancer immunotherapies designed to improve patient outcomes by unleashing the power of the immune system selectively at the site of the tumor. The company’s tumor-selective immunotherapies are based on its proprietary technology, which maximizes the potency of proven immuno-oncology therapies and restricts their activity to the tumor to minimize peripheral side effects. The broad applicability of these therapies across cancer types means that all patients could benefit from these potentially curative medicines.

Xilio was founded in 2016 and closed a $100.5M Series B financing from blue chip venture investors in February 2020. Xilio is headquartered in Waltham, MA.

To learn more, please visit www.xiliotx.com.

SUMMARY:

We are seeking an Associate Director/Director to lead our In Vivo Pharmacology group. This individual will be responsible for in vivo oncology efficacy models, and pharmacokinetic and pharmacodynamic studies for the characterization of immuno-oncology focused protein therapeutics. Management of CROs and internal staff will be required to execute on these studies.

RESPONSIBILITIES:

  • Work with Project Teams to identify the appropriate in vivo tumor models for testing the efficacy and safety of Xilio’s protein therapeutics.
  • Design and implement studies to investigate the efficacy, safety and PK/PD of protein therapeutics including analysis of data with the appropriate statistical tools
  • Oversee husbandry of mice, manage vivarium and meet all guidelines set forth by Xilio’s IACUC
  • Work with CROs, collaborators and partners to execute studies to support the discovery and optimization of protein therapeutics and to support translational studies to inform upon clinical development plans.
  • Identify and integrate technologies that improve or expand the ability to assess molecules in vivo by working with teammates, collaborators, vendors, and staying abreast of the literature
  • Ensure data is captured in appropriate databases and electronic laboratory notebooks
  • Regular participation and presentation at Project Team and departmental meetings
  • Provide proper training of staff and continue to explore opportunities to expand their responsibilities and capabilities
  • Manage team of Ph.D. and non-Ph.D. level scientists

QUALIFICATIONS:

  • Ph.D., post-doctoral experience and +10 years in the field of immuno-oncology, or other relevant fields; ideally, with 5+ years’ experience working in a CRO, biotechnology or pharmaceutical company
  • Technical experience with development, qualification, and execution of studies in syngeneic tumor models, as well as PDX and/or humanized tumor models, and IACUC documentation
  • Experience with pharmacokinetic analyses would be preferred
  • Experience managing a team
  • Project leadership experience preferred
  • xperience with histology and/or FACS analyses of in vivo models is desirable
  • Experience writing reports for regulatory documents would be helpful

POSITION: Full-Time, Exempt

EEOC Statement: Xilio Therapeutics believes in a diverse environment and is committed to equal employment opportunity for all its employees and qualified applicants. We do not discriminate in recruitment, hiring, training, promotion or any other employment practices for reasons of race, color, religion, gender, national origin, age, sexual orientation, marital or veteran status, disability, or any other legally protected status. Xilio will make reasonable accommodations for qualified individuals with known disabilities, in accordance with applicable law.

posted 2020-11-17

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Xilio Therapeutics is a development-stage biotechnology company advancing next-generation cancer immunotherapies designed to improve patient outcomes by unleashing the power of the immune system selectively at the site of the tumor. The company’s tumor-selective immunotherapies are based on its proprietary technology, which maximizes the potency of proven immuno-oncology therapies and restricts their activity to the tumor to minimize peripheral side effects. The broad applicability of these therapies across cancer types means that all patients could benefit from these potentially curative medicines.

Xilio was founded in 2016, and closed a $100.5M Series B financing from blue chip venture investors in February 2020. Xilio is headquartered in Waltham, MA.

SUMMARY:

We are seeking a Scientist to join our Protease Biology group. This person will be responsible for the development and execution of translational assays to support our immuno-oncology pipeline.

RESPONSIBILITIES:

  • Develop assays to characterize and analyze proteases expression and activity in different biological samples including primary tissue and tumor samples
  • Set up immunofluorescent techniques on tissue slides to analyze protease activity in situ and cleavage of our therapeutics
  • Devise and perform experiments to analyze cleavage of therapeutic proteins in biological matrices
  • Integrate technologies that improve or expand the ability to assay molecules by working with teammates, collaborators, CRO, and staying abreast of the literature
  • Work with the research team to provide input into translational and biochemistry studies
  • Enter all data into laboratory notebooks and databases on a daily basis and generate weekly progress reports for management review
  • Participation and presentation of data at group meetings
  • Contribute at project meetings and mentor junior scientists

REQUIREMENTS:

  • PhD in Cancer Biology / Cell Biology / Immunology, + 3 years; ideally with experience working in a biotechnology or pharmaceutical company
  • Strong scientific background on the biology of proteases in cancer
  • Technical experience in the development, qualification, and execution of assays for protease characterization in human tissues (mRNA and protein expression, protease activity measurement)
  • Experience with culture of primary human tumor and immune cells
  • xperience with fluorescence staining analysis by microscopy a plus
  • Goal oriented, organized, capable of working on multiple projects, and able to execute to timelines that meet company goals
  • Willing to learn new techniques and skills
  • Ability to contribute constructively to discussions and become a subject matter expert in new areas as needed
  • Works well in a cross functional team environment, both independently as well as be willing to help others
  • Good verbal and written communication skills
  • Driven by a desire to create the next generation of immunotherapy-based cancer therapeutics and the vision to offer cancer patients efficacious and tolerable new therapies that extend and improve their lives.

Xilio offers the opportunity to work in an innovation driven, exciting biotechnology company doing ground-breaking work in the field of immuno-oncology. We offer a competitive compensation package including a base salary, annual bonus potential, stock options, 401k, healthcare benefits and paid time-off.

POSITION: Full-Time, Exempt

EEOC Statement: Xilio Therapeutics believes in a diverse environment, and is committed to equal employment opportunity for all its employees and qualified applicants. We do not discriminate in recruitment, hiring, training, promotion or any other employment practices for reasons of race, color, religion, gender, national origin, age, sexual orientation, marital or veteran status, disability, or any other legally protected status. Xilio will make reasonable accommodations for qualified individuals with known disabilities, in accordance with applicable law.

posted 2020-11-18

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ADAM proteases as therapeutic targets - an experimental / bioinformatic approach

Our working group is dedicated to research on the function of 'a disintegrin and metalloproteinases' (ADAMs) in acute and chronic inflammatory diseases. This includes both sterile inflammation and infection. TNF and ACE2, which are related to COVID-19, are e.g. two substrates from ADAM17. In collaboration with the working group of Prof. Volkhard Helms (Center for Bioinformatics, Saarland University), we combine “wet lab” and bioinformatics in order to identify new target structures for ADAM-directed treatment.

As part of a joint initiative with the Helmholtz Institute for Infection Research (HIPS) Saarbrücken, we are looking for a highly motivated doctoral candidate for a period of three years starting in October 2020. Besides common molecular biological and biochemical methods you will use qRT-PCR, gene chip analyzes, FACS and mass spectrometry for transcriptome and proteome analysis and evaluate their data in collaboration with the Helms group. 

Qualifications:

  • MSc/equivalent for PhD in a relevant discipline (e.g. cell biology, biology, biochemistry, molecular biology, biotechnology, pharmacy, pharmacology)
  • Lab experience in cellular and molecular biology techniques.
  • Team-worker with a high grade of responsibility and flexibility

How to apply: By email at daniela.yildiz@uks.eu; send a short motivation letter, summary of master thesis, CV, contacts for two referees, transcripts. 

Further information: http://www.pzms.uni-saarland.de/index.php/jun-prof-yildiz (Jun.-Prof. Dr. rer. nat. Daniela Yildiz née Dreymueller, Saarland University, Campus Homburg); https://www-cbi.cs.uni-saarland.de (Prof. Helms)

posted 2020-08-18

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Postdoc or PhD student position in Cellular Microbiology
University of Salzburg, Austria

Position Description:
Duration: 3 years
Area: Cellular Microbiology, How Gram-positive pathogens impair E-cadherin functions

The functional integrity of the human epithelium is crucially important for the protection against intruding pathogens. The cell-adhesion molecule and signaling protein E-cadherin plays a central role in the establishment of the epithelial barrier function. Our group pioneered a novel function for the bacterial serine protease HtrA expressed by the class-I carcinogen Helicobacter pylori, which directly cleaves the ectodomain of E-cadherin. Most research was performed with HtrA expressed by Gram-negative bacteria; however, Gram-positive pathogens express a structurally different class of HtrA proteins exhibiting different specificities and activities.
In an international project together with Prof. Dr. Silja Wessler (Salzburg, Austria) and Prof. Dr. Boris Turk (Ljubljana, Slovenia), the successful candidate will investigate (i) the activation and substrate specificity of HtrA proteases expressed by selected Gram-positive pathogens and (ii) the functional role of HtrA and E-cadherin truncation in the infection process using Listeria monocytogenes and Staphylococcus aureus as model organisms. Novel methods and techniques will be employed: DIPPS (Direct In-gel Profiling of Protease Specificity), FPPS (Fast Profiling of Protease Specificity), organoids as advanced novel in vitro infection models, Next Generation Sequencing, and CRISPR/Cas9 gene editing.
Publications: Hoy et al., EMBO Rep, 2010; Schmidt et al., Sci Rep, 2016, Vidmar et al., EMBO J, 2017

Candidates:
• should have a strong background in protease biochemistry, cell and/or infection biology
• are expected to be independent, scientific-orientated and self-motivated
• have excellent communication and interpersonal skills
• collaborate with internal and external collaborators and support other group members
• employ cutting-edge technologies and standard molecular biology tools in combination with infection biology and protease function in pathogen-host interaction
• as a Postdoc: must have received the PhD within the last five years with a proven publication record (at least one first author paper).

We hold a state-of-the art laboratory at the Paris-Lodron University of Salzburg in Austria, a beautiful place at the northern boundary of the Alps. The Department of Biosciences provides broad possibilities for interaction and support. Our group is member of the international "PhD program ICA" (University of Salzburg/FWF), Cancer Cluster Salzburg (www.cancercluster-salzburg.at), and member of the priority program "Allergy-Cancer-BioNano Research Centre" (University of Salzburg).

For further information, see https://www.uni-salzburg.at/index.php?id=209601

Please send your CV, publication list and letter of interest to Silja Wessler (silja.wessler@sbg.ac.at). The duration of the position is three years with the possibility of extension. Closing date: October 31, 2019

posted 2019-10-09

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Postdoc Scholarship in Biochemistry
UNIFESP-EPM, São Paulo Brazil

The laboratory of Protein Chemistry, Department of Biochemistry, UNIFESP-EPM, offers a (01) postdoc position related to the thematic project “Fragments derived from the structure of protease inhibitors with selectivity for inhibition of mammalian and microorganism enzymes and its role as an anti-inflammatory, antimicrobial, antithrombotic and anti-tumor agent: mechanism of action” coordinated by Prof. Dr. Maria Luiza Vilela Oliva.
The candidate will participate in research of cell/molecular biology and biochemistry investigating the action of compounds in tumor and inflammation models in vitro and in vivo. The postdoc must have experience on protein and peptide characterization and purification, aiming at the isolation of samples used in the assays described above. The candidate must be fluent in English and have a PhD in Biological Sciences, with emphasis on Cellular / Molecular Biology and Biochemistry, in a maximum period of 7 years.

Skills and experience required for selection:
• Protein / peptides characterization and purification by chromatographic techniques, FPLC and HPLC (fundamental);
• Experience of cellular behavior by confocal microscopy (desirable);
• Flow cytometry technique (fundamental);
• Manipulation with cell lines and 3D culture (fundamental);
• Experience with real time PCR and western blotting technique (fundamental);
• Knowledge and experience in animal manipulation and experimentation (fundamental);
• Evaluation of leukocyte behavior by intravital microscopy (fundamental);
• Analysis of proinflammatory cytokines and determination of nitric oxide (desirable);
• Experiences in heterologous protein expression (desirable);
• Fluency in English with experience of at least 6 months abroad (fundamental);
• Written and oral communication skills (fundamental).

Duration: Initially 24 months, renewable up to 48 months, depending on results obtained and reports delivered every 12 months. The postdoctoral fellowship includes a monthly fee of R $ 7,373.10 and technical research reserve (15% of the annual value of the scholarship, each year). For more details, check the FAPESP website: www.fapesp.br/bolsas/pd.

Subscription: Interested in the vacancy should contact Prof. Dr. Maria Luiza Vilela Oliva (mlvoliva@unifesp.br) and must contain:
1. Motivation letter (previous experience, containing technical skills, and interest in the position);
2. Curriculum Vitae;
3. Letter of recommendation from three professors for academic reference, at least one of them foreign.
Applicants must submit the attached and combined documentation in a single PDF.

Selection criteria: a) Analysis of the curriculum, regarding the academic formation directed to execution of the research project; being necessary the skills requested above.
b) Interview (personal or via Skype), if necessary, to be scheduled with the candidate.
c) Possible written test to evaluate the knowledge in the requested skills.

posted 2019-08-29

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Postdoctoral Position in Proteomics and Protease Biology
University of British Columbia, Vancouver, Canada

Position Description
Area: Protease biology and proteomics
Duration: 1 year; based on performance can be extended for up to 3 years
Expected starting date: Application review will begin immediately and will continue until the position is filled.
Starting annual stipend: CAD 45,000/yr plus benefits

We are seeking a highly motivated, enthusiastic and independently thinking post-doctoral candidate studying the cellular ligase activities of protease. The candidate will work under the direct supervision of Dr. Dieter Brömme and will use cellular, biochemical, protein chemistry, and mass-spectrometry-based proteomics approaches. The candidate will be part of collaborative team. The minimum academic requirement is Ph.D. in a life science subject that should not be older than 2 years.

Qualifications needed:
• Candidate must have extensive experience in various proteomics technologies including been capable to perform LC-tandem mass spectrometry techniques (LC-MS/MS) and the appropriate data analysis.
• Candidate must have extensive expertise in cell culture techniques (cell-lines and primary cells).
• Candidate is required to have expertise in various protein separation techniques (FPLC, HPLC, other chromatography techniques, electrophoresis, immunoprecipitation).
• The candidate has excellent verbal and written skills in English and has demonstrated the capability to write research protocols and papers as well as to maintain meticulous research records.
• Candidate must be independent yet collaborative, self-motivated, capable of creative thinking, and driven with a desire to pursue a career as an independent academic scientist.

Instructions
• Cover letter outlining your research interest and suitability for the position
• CV
• At least 2 first author paper examples and track-record of international conference presentations
• Names and contact information of 3 references

Applications will be sent to:
Dieter Brömme, Ph.D.
Professor and Canada Research Chair in Protease and Diseases
The University of British Columbia
Email: dbromme@dentistry.ubc.ca

The University of British Columbia (UBC) is a global centre for teaching, learning and research, consistently ranked among the top 20 public universities in the world and recently recognized as North America’s most international university.

posted 2019-08-04

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Postdoctoral Fellow Position in Drug Development
University of British Columbia, Vancouver, Canada

Position Description
Area: Musculo-skleletal disease or skin aging models and drug development
Duration: 1 year; based on performance can be extended for up to 3 years
Expected starting date: Application review will begin immediately and will continue until the position is filled.
Starting annual stipend: CAD 45,000/yr plus benefits

We are seeking a highly motivated, enthusiastic and independently thinking post-doctoral candidate studying novel approaches to develop multifunctional drug therapies for musculoskeletal diseases an/or skin ageing. The candidate will work under the direct supervision of Dr. Dieter Brömme and will use cellular, biochemical, and various imaging approaches (Confocal/SEM/TEM/µCT). The candidate will be part of collaborative team. The minimum academic requirement is Ph.D. in a life science/medical science subject that should not be older than 2-3 years.

Qualifications needed:
• Candidates must have extensive experience in biochemistry, cell biology, musculoskeletal or skin ageing biology, and rodent disease models.
• Candidate must have extensive experience in drug application methods (oral, injectable, topical), animal care and record keeping, blood withdrawal and animal dissecting skills.
• Candidate is required to have histology and immunohistochemistry skills.
• Hands-on experience in various imaging (µCT, confocal time-lapse, SEM) and cell-sorting techniques is expected.
• Some experience in pharmacokinetic techniques and analysis would be beneficial.
• The candidate has excellent verbal and written skills in English and has demonstrated the capability to write research protocols and papers as well as to maintain meticulous research records.
• Candidate must be independent yet collaborative, self-motivated, capable of creative thinking, and driven with a desire to pursue a career as an independent academic scientist or a career in a drug development company.

Instructions
• Cover letter outlining your research interest and suitability for the position
• CV
• At least 2 first author paper examples and track-record of international conference presentations.
• Names and contact information of 3 references

Applications will be sent to:
Dieter Brömme, Ph.D.
Professor and Canada Research Chair in Protease and Diseases
The University of British Columbia
Email: dbromme@dentistry.ubc.ca

The University of British Columbia (UBC) is a global centre for teaching, learning and research, consistently ranked among the top 20 public universities in the world and recently recognized as North America’s most international university.

posted 2019-08-04

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M.Sc., Ph.D., and PDF positions
University of Sherbrooke (Québec), Canada

Seeking highly motivated graduate students and PDFs to investigate the interplay of caspases and cancer

The Denault’s laboratory is seeking MSc, PhD, and PDF candidates with interest in the interplay of caspases and cancer, more specifically, how caspase-mediated proteolysis influences growth factor signaling in colorectal cancer (CRC). The position is available immediately at the Institut de Pharmacologie de Sherbrooke (IPS; yes another IPS!) of the University of Sherbrooke (Québec), Canada. MSc and PhD trainees will enroll into the graduate program in pharmacology.

The available projects will require working with cell cultures, tissues from patients, several techniques of molecular biology (siRNA, CRISPR, lentivirus), cell biology (confocal and STED microscopy, subcellular fractionation), biochemistry (enzymology, protein-protein interaction analysis) as well as proteomics.

The lab is part of the Department of Pharmacology and Physiology at the IPS, in Sherbrooke, Province of Québec, Canada. Sherbrooke is a university town nestled in the beautiful Eastern Townships of the Province, surrounded by mountains, rivers, and lakes.

Candidates will benefit from the complementary expertise of researchers at the Department as well as their close collaborators from other departments and institutions. Our Institute has state of the art facilities including STED and light sheet microscopy, NMR for protein structure, -omics, small animal imaging, and more, and is situated on the same campus as a medical school hospital and a clinical research center. Aside from lab work and the few courses, trainees will have the opportunity to learn French; attend training classes in scientific writing, IP, career building, teaching, and more; make new friends; and enjoy the Canadian outdoors.

Qualifications:

We are looking for an enthusiastic, hard-working, motivated, rigorous, and interactive person with keen interest in proteolysis, cancer biology, and intracellular trafficking, and the following qualifications:

• Undergraduate degree for MSc candidates, MSc/equivalent for PhD, or PhD for PDF, in a relevant discipline (cell biology, biochemistry, molecular biology, biotechnology, etc.)
• Excellent academic track record;
• Publications in peer-reviewed journals for candidates with a PhD degree;
• Some lab experience in cellular and molecular biology techniques.

How to apply: By email at jean-bernard.denault@USherbrooke.ca; send a short motivation letter, CV, contacts for two referees, and latest (non-official) transcripts for M.Sc./Ph.D. candidates.

posted 2019-03-11

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