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Below are our current protease-related job listings:

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Postdoc or PhD student position in Cellular Microbiology
University of Salzburg, Austria

Position Description:
Duration: 3 years
Area: Cellular Microbiology, How Gram-positive pathogens impair E-cadherin functions

The functional integrity of the human epithelium is crucially important for the protection against intruding pathogens. The cell-adhesion molecule and signaling protein E-cadherin plays a central role in the establishment of the epithelial barrier function. Our group pioneered a novel function for the bacterial serine protease HtrA expressed by the class-I carcinogen Helicobacter pylori, which directly cleaves the ectodomain of E-cadherin. Most research was performed with HtrA expressed by Gram-negative bacteria; however, Gram-positive pathogens express a structurally different class of HtrA proteins exhibiting different specificities and activities.
In an international project together with Prof. Dr. Silja Wessler (Salzburg, Austria) and Prof. Dr. Boris Turk (Ljubljana, Slovenia), the successful candidate will investigate (i) the activation and substrate specificity of HtrA proteases expressed by selected Gram-positive pathogens and (ii) the functional role of HtrA and E-cadherin truncation in the infection process using Listeria monocytogenes and Staphylococcus aureus as model organisms. Novel methods and techniques will be employed: DIPPS (Direct In-gel Profiling of Protease Specificity), FPPS (Fast Profiling of Protease Specificity), organoids as advanced novel in vitro infection models, Next Generation Sequencing, and CRISPR/Cas9 gene editing.
Publications: Hoy et al., EMBO Rep, 2010; Schmidt et al., Sci Rep, 2016, Vidmar et al., EMBO J, 2017

Candidates:
• should have a strong background in protease biochemistry, cell and/or infection biology
• are expected to be independent, scientific-orientated and self-motivated
• have excellent communication and interpersonal skills
• collaborate with internal and external collaborators and support other group members
• employ cutting-edge technologies and standard molecular biology tools in combination with infection biology and protease function in pathogen-host interaction
• as a Postdoc: must have received the PhD within the last five years with a proven publication record (at least one first author paper).

We hold a state-of-the art laboratory at the Paris-Lodron University of Salzburg in Austria, a beautiful place at the northern boundary of the Alps. The Department of Biosciences provides broad possibilities for interaction and support. Our group is member of the international "PhD program ICA" (University of Salzburg/FWF), Cancer Cluster Salzburg (www.cancercluster-salzburg.at), and member of the priority program "Allergy-Cancer-BioNano Research Centre" (University of Salzburg).

For further information, see https://www.uni-salzburg.at/index.php?id=209601

Please send your CV, publication list and letter of interest to Silja Wessler (silja.wessler@sbg.ac.at). The duration of the position is three years with the possibility of extension. Closing date: October 31, 2019

posted 2019-10-09

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Postdoc Scholarship in Biochemistry
UNIFESP-EPM, São Paulo Brazil

The laboratory of Protein Chemistry, Department of Biochemistry, UNIFESP-EPM, offers a (01) postdoc position related to the thematic project “Fragments derived from the structure of protease inhibitors with selectivity for inhibition of mammalian and microorganism enzymes and its role as an anti-inflammatory, antimicrobial, antithrombotic and anti-tumor agent: mechanism of action” coordinated by Prof. Dr. Maria Luiza Vilela Oliva.
The candidate will participate in research of cell/molecular biology and biochemistry investigating the action of compounds in tumor and inflammation models in vitro and in vivo. The postdoc must have experience on protein and peptide characterization and purification, aiming at the isolation of samples used in the assays described above. The candidate must be fluent in English and have a PhD in Biological Sciences, with emphasis on Cellular / Molecular Biology and Biochemistry, in a maximum period of 7 years.

Skills and experience required for selection:
• Protein / peptides characterization and purification by chromatographic techniques, FPLC and HPLC (fundamental);
• Experience of cellular behavior by confocal microscopy (desirable);
• Flow cytometry technique (fundamental);
• Manipulation with cell lines and 3D culture (fundamental);
• Experience with real time PCR and western blotting technique (fundamental);
• Knowledge and experience in animal manipulation and experimentation (fundamental);
• Evaluation of leukocyte behavior by intravital microscopy (fundamental);
• Analysis of proinflammatory cytokines and determination of nitric oxide (desirable);
• Experiences in heterologous protein expression (desirable);
• Fluency in English with experience of at least 6 months abroad (fundamental);
• Written and oral communication skills (fundamental).

Duration: Initially 24 months, renewable up to 48 months, depending on results obtained and reports delivered every 12 months. The postdoctoral fellowship includes a monthly fee of R $ 7,373.10 and technical research reserve (15% of the annual value of the scholarship, each year). For more details, check the FAPESP website: www.fapesp.br/bolsas/pd.

Subscription: Interested in the vacancy should contact Prof. Dr. Maria Luiza Vilela Oliva (mlvoliva@unifesp.br) and must contain:
1. Motivation letter (previous experience, containing technical skills, and interest in the position);
2. Curriculum Vitae;
3. Letter of recommendation from three professors for academic reference, at least one of them foreign.
Applicants must submit the attached and combined documentation in a single PDF.

Selection criteria: a) Analysis of the curriculum, regarding the academic formation directed to execution of the research project; being necessary the skills requested above.
b) Interview (personal or via Skype), if necessary, to be scheduled with the candidate.
c) Possible written test to evaluate the knowledge in the requested skills.

posted 2019-08-29

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Postdoctoral Position in Proteomics and Protease Biology
University of British Columbia, Vancouver, Canada

Position Description
Area: Protease biology and proteomics
Duration: 1 year; based on performance can be extended for up to 3 years
Expected starting date: Application review will begin immediately and will continue until the position is filled.
Starting annual stipend: CAD 45,000/yr plus benefits

We are seeking a highly motivated, enthusiastic and independently thinking post-doctoral candidate studying the cellular ligase activities of protease. The candidate will work under the direct supervision of Dr. Dieter Brömme and will use cellular, biochemical, protein chemistry, and mass-spectrometry-based proteomics approaches. The candidate will be part of collaborative team. The minimum academic requirement is Ph.D. in a life science subject that should not be older than 2 years.

Qualifications needed:
• Candidate must have extensive experience in various proteomics technologies including been capable to perform LC-tandem mass spectrometry techniques (LC-MS/MS) and the appropriate data analysis.
• Candidate must have extensive expertise in cell culture techniques (cell-lines and primary cells).
• Candidate is required to have expertise in various protein separation techniques (FPLC, HPLC, other chromatography techniques, electrophoresis, immunoprecipitation).
• The candidate has excellent verbal and written skills in English and has demonstrated the capability to write research protocols and papers as well as to maintain meticulous research records.
• Candidate must be independent yet collaborative, self-motivated, capable of creative thinking, and driven with a desire to pursue a career as an independent academic scientist.

Instructions
• Cover letter outlining your research interest and suitability for the position
• CV
• At least 2 first author paper examples and track-record of international conference presentations
• Names and contact information of 3 references

Applications will be sent to:
Dieter Brömme, Ph.D.
Professor and Canada Research Chair in Protease and Diseases
The University of British Columbia
Email: dbromme@dentistry.ubc.ca

The University of British Columbia (UBC) is a global centre for teaching, learning and research, consistently ranked among the top 20 public universities in the world and recently recognized as North America’s most international university.

posted 2019-08-04

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Postdoctoral Fellow Position in Drug Development
University of British Columbia, Vancouver, Canada

Position Description
Area: Musculo-skleletal disease or skin aging models and drug development
Duration: 1 year; based on performance can be extended for up to 3 years
Expected starting date: Application review will begin immediately and will continue until the position is filled.
Starting annual stipend: CAD 45,000/yr plus benefits

We are seeking a highly motivated, enthusiastic and independently thinking post-doctoral candidate studying novel approaches to develop multifunctional drug therapies for musculoskeletal diseases an/or skin ageing. The candidate will work under the direct supervision of Dr. Dieter Brömme and will use cellular, biochemical, and various imaging approaches (Confocal/SEM/TEM/µCT). The candidate will be part of collaborative team. The minimum academic requirement is Ph.D. in a life science/medical science subject that should not be older than 2-3 years.

Qualifications needed:
• Candidates must have extensive experience in biochemistry, cell biology, musculoskeletal or skin ageing biology, and rodent disease models.
• Candidate must have extensive experience in drug application methods (oral, injectable, topical), animal care and record keeping, blood withdrawal and animal dissecting skills.
• Candidate is required to have histology and immunohistochemistry skills.
• Hands-on experience in various imaging (µCT, confocal time-lapse, SEM) and cell-sorting techniques is expected.
• Some experience in pharmacokinetic techniques and analysis would be beneficial.
• The candidate has excellent verbal and written skills in English and has demonstrated the capability to write research protocols and papers as well as to maintain meticulous research records.
• Candidate must be independent yet collaborative, self-motivated, capable of creative thinking, and driven with a desire to pursue a career as an independent academic scientist or a career in a drug development company.

Instructions
• Cover letter outlining your research interest and suitability for the position
• CV
• At least 2 first author paper examples and track-record of international conference presentations.
• Names and contact information of 3 references

Applications will be sent to:
Dieter Brömme, Ph.D.
Professor and Canada Research Chair in Protease and Diseases
The University of British Columbia
Email: dbromme@dentistry.ubc.ca

The University of British Columbia (UBC) is a global centre for teaching, learning and research, consistently ranked among the top 20 public universities in the world and recently recognized as North America’s most international university.

posted 2019-08-04

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M.Sc., Ph.D., and PDF positions
University of Sherbrooke (Québec), Canada

Seeking highly motivated graduate students and PDFs to investigate the interplay of caspases and cancer

The Denault’s laboratory is seeking MSc, PhD, and PDF candidates with interest in the interplay of caspases and cancer, more specifically, how caspase-mediated proteolysis influences growth factor signaling in colorectal cancer (CRC). The position is available immediately at the Institut de Pharmacologie de Sherbrooke (IPS; yes another IPS!) of the University of Sherbrooke (Québec), Canada. MSc and PhD trainees will enroll into the graduate program in pharmacology.

The available projects will require working with cell cultures, tissues from patients, several techniques of molecular biology (siRNA, CRISPR, lentivirus), cell biology (confocal and STED microscopy, subcellular fractionation), biochemistry (enzymology, protein-protein interaction analysis) as well as proteomics.

The lab is part of the Department of Pharmacology and Physiology at the IPS, in Sherbrooke, Province of Québec, Canada. Sherbrooke is a university town nestled in the beautiful Eastern Townships of the Province, surrounded by mountains, rivers, and lakes.

Candidates will benefit from the complementary expertise of researchers at the Department as well as their close collaborators from other departments and institutions. Our Institute has state of the art facilities including STED and light sheet microscopy, NMR for protein structure, -omics, small animal imaging, and more, and is situated on the same campus as a medical school hospital and a clinical research center. Aside from lab work and the few courses, trainees will have the opportunity to learn French; attend training classes in scientific writing, IP, career building, teaching, and more; make new friends; and enjoy the Canadian outdoors.

Qualifications:

We are looking for an enthusiastic, hard-working, motivated, rigorous, and interactive person with keen interest in proteolysis, cancer biology, and intracellular trafficking, and the following qualifications:

• Undergraduate degree for MSc candidates, MSc/equivalent for PhD, or PhD for PDF, in a relevant discipline (cell biology, biochemistry, molecular biology, biotechnology, etc.)
• Excellent academic track record;
• Publications in peer-reviewed journals for candidates with a PhD degree;
• Some lab experience in cellular and molecular biology techniques.

How to apply: By email at jean-bernard.denault@USherbrooke.ca; send a short motivation letter, CV, contacts for two referees, and latest (non-official) transcripts for M.Sc./Ph.D. candidates.

posted 2019-03-11

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